4/30/2023 0 Comments Inside out patch clamp methodThere are many types of glass pipettes available. Lesson learned – not all pipettes are created equal! After a frustrating day of getting nowhere, my mentor stepped in to help and promptly laughed at my pipettes. I still remember during the first month of my PhD learning about the importance of choosing the right material for a patch-clamp pipette. Here are a few tips on preparing the right pipettes for the job, which will hopefully convert your patching from a frustrating experience to a satisfying one. You then “pull” a capillary using a programmable puller to obtain two tip-shaped pipettes that can then be used for the experiment. Pipettes for patch clamping are supplied by companies as glass capillaries. While the premise is simple, the process of obtaining a seal with your patch clamping pipette can be more of an obscure art that requires, among other things, the ability to craft your own pipette. The patch clamping technique is based on a simple premise: the formation of a strong seal between the membrane of a cell or artificial bilayer and a tiny portion of a pipette tip this is called a gigaseal. The patch-clamp technique was first developed by Neher and Sakmann in 1976 and is used to measure either the currents passing through ion channels (voltage-clamp) or the membrane potential (current-clamp) of electrically active cells.
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